dc.contributor.author | Berg, Sigrid | |
dc.contributor.author | Eggen, Siv | |
dc.contributor.author | Caidahl, Kenneth | |
dc.contributor.author | Dähne, Lars | |
dc.contributor.author | Hansen, Rune | |
dc.date.accessioned | 2023-02-23T12:10:31Z | |
dc.date.available | 2023-02-23T12:10:31Z | |
dc.date.created | 2022-12-02T09:29:45Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | PLOS ONE. 2022, 17 (11), e0276292. | en_US |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | https://hdl.handle.net/11250/3053589 | |
dc.description.abstract | Contrast enhanced ultrasound is a powerful diagnostic tool and ultrasound contrast media are based on microbubbles (MBs). The use of MBs in drug delivery applications and molecular imaging is a relatively new field of research which has gained significant interest during the last decade. MBs available for clinical use are fragile with short circulation half-lives due to the use of a thin encapsulating shell for stabilization of the gas core. Thick-shelled MBs can have improved circulation half-lives, incorporate larger amounts of drugs for enhanced drug delivery or facilitate targeting for use in molecular ultrasound imaging. However, methods for robust imaging of thick-shelled MBs are currently not available. We propose a simple multi-pulse imaging technique which is able to visualize thick-shelled polymeric MBs with a superior contrast-to-tissue ratio (CTR) compared to commercially available harmonic techniques. The method is implemented on a high-end ultrasound scanner and in-vitro imaging in a tissue mimicking flow phantom results in a CTR of up to 23 dB. A proof-of-concept study of molecular ultrasound imaging in a soft tissue inflammation model in rabbit is then presented where the new imaging technique showed an enhanced accumulation of targeted MBs in the inflamed tissue region compared to non-targeted MBs and a mean CTR of 13.3 dB for stationary MBs. The presence of fluorescently labelled MBs was verified by confocal microscopy imaging of tissue sections post-mortem. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | PLOS | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | A multi-pulse ultrasound technique for imaging of thick-shelled microbubbles demonstrated in vitro and in vivo | en_US |
dc.title.alternative | A multi-pulse ultrasound technique for imaging of thick-shelled microbubbles demonstrated in vitro and in vivo | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | © 2022 Berg et al. | en_US |
dc.source.pagenumber | 19 | en_US |
dc.source.volume | 17 | en_US |
dc.source.journal | PLOS ONE | en_US |
dc.source.issue | 11 | en_US |
dc.identifier.doi | 10.1371/journal.pone.0276292 | |
dc.identifier.cristin | 2087567 | |
dc.relation.project | Norges forskningsråd: 240410 | en_US |
dc.source.articlenumber | e0276292 | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |