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dc.contributor.authorSporre, Emil
dc.contributor.authorKarlsen, Jan
dc.contributor.authorSchriever, Karen
dc.contributor.authorAsplund-Samuelsson, Johannes
dc.contributor.authorJanasch, Markus
dc.contributor.authorStrandberg, Linnéa
dc.contributor.authorKarlsson, Anna
dc.contributor.authorKotol, David
dc.contributor.authorZeckey, Luise
dc.contributor.authorPiazza, Ilaria
dc.contributor.authorSyrén, Per-Olof
dc.contributor.authorEdfors, Fredrik
dc.contributor.authorHudson, Elton P.
dc.identifier.citationCommunications Biology. 2023, 6, 1-15: 947.en_US
dc.description.abstractMetabolite-level regulation of enzyme activity is important for microbes to cope with environmental shifts. Knowledge of such regulations can also guide strain engineering for biotechnology. Here we apply limited proteolysis-small molecule mapping (LiP-SMap) to identify and compare metabolite-protein interactions in the proteomes of two cyanobacteria and two lithoautotrophic bacteria that fix CO2 using the Calvin cycle. Clustering analysis of the hundreds of detected interactions shows that some metabolites interact in a species-specific manner. We estimate that approximately 35% of interacting metabolites affect enzyme activity in vitro, and the effect is often minor. Using LiP-SMap data as a guide, we find that the Calvin cycle intermediate glyceraldehyde-3-phosphate enhances activity of fructose-1,6/sedoheptulose-1,7-bisphosphatase (F/SBPase) from Synechocystis sp. PCC 6803 and Cupriavidus necator in reducing conditions, suggesting a convergent feed-forward activation of the cycle. In oxidizing conditions, glyceraldehyde-3-phosphate inhibits Synechocystis F/SBPase by promoting enzyme aggregation. In contrast, the glycolytic intermediate glucose-6-phosphate activates F/SBPase from Cupriavidus necator but not F/SBPase from Synechocystis. Thus, metabolite-level regulation of the Calvin cycle is more prevalent than previously appreciated.en_US
dc.publisherSpringer Natureen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleMetabolite interactions in the bacterial Calvin cycle and implications for flux regulationen_US
dc.title.alternativeMetabolite interactions in the bacterial Calvin cycle and implications for flux regulationen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.rights.holder© The Author(s) 2023. Published by Springer Nature.en_US
dc.source.journalCommunications Biologyen_US

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