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dc.contributor.authorNgoumelah, Daniel Dzofou
dc.contributor.authorHarnisch, Falk
dc.contributor.authorSulheim, Snorre
dc.contributor.authorHeggeset, Tonje Marita Bjerkan
dc.contributor.authorAune, Ingvild Haugnes
dc.contributor.authorWentzel, Alexander
dc.contributor.authorKretzschmar, Jörg
dc.identifier.citationFrontiers in Microbiology, 2023, 13,en_US
dc.description.abstractApart from their archetypic use in anaerobic digestion (AD) methanogenic archaea are targeted for a wide range of applications. Using different methanogenic archaea for one specific application requires the optimization of culture media to enable the growth of different strains under identical environmental conditions, e.g., in microbial electrochemical technologies (MET) for (bio)electromethanation. Here we present a new culture medium (BFS01) adapted from the DSM-120 medium by omitting resazurin, yeast extract, casitone, and using a low salt concentration, that was optimized for Methanosarcina barkeri, Methanobacterium formicicum, and Methanothrix soehngenii. The aim was to provide a medium for follow-up co-culture studies using specific methanogens and Geobacter spp. dominated biofilm anodes. All three methanogens showed growth and activity in the BFS01 medium. This was demonstrated by estimating the specific growth rates ( μ ) and doubling times ( td ) of each methanogen. The μ and td based on methane accumulation in the headspace showed values consistent with literature values for M. barkeri and M. soehngenii. However, μ and td based on methane accumulation in the headspace differed from literature data for M. formicicum but still allowed sufficient growth. The lowered salt concentration and the omission of chemically complex organic components in the medium may have led to the observed deviation from μ and td for M. formicicum as well as the changed morphology. 16S rRNA gene-based amplicon sequencing and whole genome nanopore sequencing further confirmed purity and species identity.en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.subjectspecific growth rateen_US
dc.subjectdoubling timeen_US
dc.subjectbioelectrochemical systemsen_US
dc.subjectmicrobial electrochemical technologyen_US
dc.subjectmedium adaptationen_US
dc.subjectmethanogenic archaeaen_US
dc.titleA unified and simple medium for growing model methanogensen_US
dc.title.alternativeA unified and simple medium for growing model methanogensen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.rights.holder© 2023 Dzofou Ngoumelah, Harnisch, Sulheim, Heggeset, Aune, Wentzel and Kretzschmar.en_US
dc.subject.nsiVDP::Bioteknologi: 590en_US
dc.subject.nsiVDP::Biotechnology: 590en_US
dc.subject.nsiVDP::Bioteknologi: 590en_US
dc.subject.nsiVDP::Biotechnology: 590en_US
dc.source.journalFrontiers in Microbiologyen_US

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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal