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dc.contributor.authorMarzi, Anne
dc.contributor.authorEder, Kai Moritz
dc.contributor.authorBarroso, Álvaro
dc.contributor.authorWågbø, Ane Marit
dc.contributor.authorMørch, Ýrr Asbjørg
dc.contributor.authorHatletveit, Anne Rein
dc.contributor.authorVisnes, Torkild
dc.contributor.authorSchmid, Ruth Baumberger
dc.contributor.authorKlinkenberg, Geir
dc.contributor.authorKemper, Björn
dc.contributor.authorSchnekenburger, Jürgen
dc.identifier.citationDrug Delivery and Translational Research. 2022, 12 (9), 2207-2224.en_US
dc.description.abstractState-of-the-art in vitro test systems for nanomaterial toxicity assessment are based on dyes and several staining steps which can be affected by nanomaterial interference. Digital holographic microscopy (DHM), an interferometry-based variant of quantitative phase imaging (QPI), facilitates reliable proliferation quantification of native cell populations and the extraction of morphological features in a fast and label- and interference-free manner by biophysical parameters. DHM therefore has been identified as versatile tool for cytotoxicity testing in biomedical nanotechnology. In a comparative study performed at two collaborating laboratories, we investigated the interlaboratory variability and performance of DHM in nanomaterial toxicity testing, utilizing complementary standard operating procedures (SOPs). Two identical custom-built off-axis DHM systems, developed for usage in biomedical laboratories, equipped with stage-top incubation chambers were applied at different locations in Europe. Temporal dry mass development, 12-h dry mass increments and morphology changes of A549 human lung epithelial cell populations upon incubation with two variants of poly(alkyl cyanoacrylate) (PACA) nanoparticles were observed in comparison to digitonin and cell culture medium controls. Digitonin as cytotoxicity control, as well as empty and cabazitaxel-loaded PACA nanocarriers, similarly impacted 12-h dry mass development and increments as well as morphology of A549 cells at both participating laboratories. The obtained DHM data reflected the cytotoxic potential of the tested nanomaterials and are in agreement with corresponding literature on biophysical and chemical assays. Our results confirm DHM as label-free cytotoxicity assay for polymeric nanocarriers as well as the repeatability and reproducibility of the technology. In summary, the evaluated DHM assay could be efficiently implemented at different locations and facilitates interlaboratory in vitro toxicity testing of nanoparticles with prospects for application in regulatory science.en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.subjectTechnology transferen_US
dc.subjectRegulatory scienceen_US
dc.subjectIn vitroen_US
dc.subjectInterlaboratory comparisonen_US
dc.subjectLabel-free cytotoxicity testingen_US
dc.subjectQuantitative phase imagingen_US
dc.subjectDigital holographic microscopyen_US
dc.titleInterlaboratory evaluation of a digital holographic microscopy–based assay for label-free in vitro cytotoxicity testing of polymeric nanocarriersen_US
dc.title.alternativeInterlaboratory evaluation of a digital holographic microscopy–based assay for label-free in vitro cytotoxicity testing of polymeric nanocarriersen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.rights.holder© The Author(s) 2022en_US
dc.source.journalDrug Delivery and Translational Researchen_US

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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal