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dc.contributor.authorPerugini, Valeria
dc.contributor.authorSchmid, Ruth
dc.contributor.authorMørch, Ýrr Asbjørg
dc.contributor.authorTexier, Isabelle
dc.contributor.authorBrodde, Martin
dc.contributor.authorSantin, Matteo
dc.identifier.citationDrug Delivery and Translational Research. 2022, 1-12 .en_US
dc.description.abstractThe development of drug nanocarriers based on polymeric, lipid and ceramic biomaterials has been paving the way to precision medicine, where the delivery of poorly soluble active compounds and personalized doses are made possible. However, the nano-size character of these carriers has been demonstrated to have the potential to elicit pathways of the host response different from those of the same biomaterials when engineered as larger size implants and of the drugs when administered without a carrier. Therefore, a specific regulatory framework needs to be made available that can offer robust scientific insights and provide safety data by reliable tests of these novel nano-devices. In this context, the present work presents a multistep protocol for the in vitro assessment of the hemocompatibility of nanocarriers of different physicochemical properties. Poly (ethyl butyl cyanoacrylate) nanoparticles and lipid-based (LipImage™ 815) nanoparticles of comparable hydrodynamic diameter were tested through a battery of assays using human peripheral blood samples and recapitulating the main pathways of the host response upon systemic administration; i.e., protein interactions, fibrinogen-platelet binding, cytotoxicity, and inflammatory response. The data showed the sensitivity and reproducibility of the methods adopted that were also demonstrated to determine individual variability as well as to discriminate between activation of pathways of inflammation and unintended release of inflammatory signaling caused by loss of cell integrity. Therefore, this multistep testing is proposed as a reliable protocol for nanoparticle development and emerging regulatory frameworks.en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.subjectInflammatory responseen_US
dc.subjectProtein coronaen_US
dc.subjectHost responseen_US
dc.subjectIn vitro testsen_US
dc.subjectLipid nanoparticlesen_US
dc.subjectPolymeric nanoparticlesen_US
dc.subjectDrug nanocarriersen_US
dc.titleA multistep in vitro hemocompatibility testing protocol recapitulating the foreign body reaction to nanocarriersen_US
dc.title.alternativeA multistep in vitro hemocompatibility testing protocol recapitulating the foreign body reaction to nanocarriersen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.rights.holderCopyright © 2022, The Author(s)en_US
dc.source.journalDrug Delivery and Translational Researchen_US

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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal