Browsing SINTEF Open by Author "Wentzel, Alexander"
Now showing items 1-20 of 24
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Automatic reconstruction of metabolic pathways from identified biosynthetic gene clusters
Sulheim, Snorre; Fossheim, Fredrik A.; Wentzel, Alexander; Almaas, Eivind (Peer reviewed; Journal article, 2021)A wide range of bioactive compounds is produced by enzymes and enzymatic complexes encoded in biosynthetic gene clusters (BGCs). These BGCs can be identified and functionally annotated based on their DNA sequence. Candidates ... -
Butanol production from lignocellulosic biomass: revisiting fermentation performance indicators with exploratory data analysis
Birgen, Cansu; Dürre, Peter; Preisig, Heinz A.; Wentzel, Alexander (Journal article; Peer reviewed, 2019)After just more than 100 years of history of industrial acetone–butanol–ethanol (ABE) fermentation, patented by Weizmann in the UK in 1915, butanol is again today considered a promising biofuel alternative based on several ... -
Butanol production from lignocellulosic sugars by Clostridium beijerinckii in microbioreactors
Birgen, Cansu; Degnes, Kristin Fløgstad; Markussen, Sidsel; Wentzel, Alexander; Sletta, Håvard (Peer reviewed; Journal article, 2021)Butanol (n-butanol) has been gaining attention as a renewable energy carrier and an alternative biofuel with superior properties to the most widely used ethanol. We performed 48 anaerobic fermentations simultaneously with ... -
Effect of Elevated Hydrogen Partial Pressure on Mixed Culture Homoacetogenesis
Sivalingam, Vasan; Haugen, Tone; Wentzel, Alexander; Dinamarca, Carlos (Peer reviewed; Journal article, 2021)This study aimed to systematically investigate the effect of elevated hydrogen partial pressure on mixed culture homoacetogenesis in the range of 1–25 bar. Seven batch experiments were performed at different initial headspace ... -
The Effect of Feeding Strategy on Butanol Production by Clostridium beijerinckii NCIMB 8052 Using Glucose and Xylose
Birgen, Cansu; Markussen, Sidsel; Wentzel, Alexander; Preisig, Heinz A. (Peer reviewed; Journal article, 2018)We performed fed-batch fermentations of glucose and xylose mixtures producing butanol. Our aim was to develop a feeding strategy for coping with carbon catabolite repression (CCR) and sequential utilization problems as ... -
Efficacy of a novel sequential enzymatic hydrolysis of lignocellulosic biomass and inhibition characteristics of monosugars
Baksi, Sibashish; Ball, Akash K.; Sarkar, Ujjaini; Banerjee, Debopam; Wentzel, Alexander; Preisig, Heinz A.; Kuniyal, Jagdish Chandra; Birgen, Cansu; Saha, Sudeshna; Wittgens, Bernd; Markussen, Sidsel (Peer reviewed; Journal article, 2019)Efficient production of sugar monomers from lignocellulose is often hampered by serious bottle-necks in biomass hydrolysis. The present study reveals that ultra-sonication assisted pretreatment following autoclaving, termed ... -
Enzyme-Constrained Models and Omics Analysis of Streptomyces coelicolor Reveal Metabolic Changes that Enhance Heterologous Production
Sulheim, Snorre; Kumelj, Tjasa; van Dissel, Dino; Salehzadeh-Yazdi, Ali; Chao, Du; Van Wezel, Gilles P.; Nieselt, Kay; Almaas, Eivind; Wentzel, Alexander; Kerkhoven, Eduard J (Peer reviewed; Journal article, 2020)Many biosynthetic gene clusters (BGCs) require heterologous expression to realize their genetic potential, including silent and metagenomic BGCs. Although the engineered Streptomyces coelicolor M1152 is a widely used host ... -
Flow-based method for biofilm microbiota enrichment and exploration of metagenomes
Hageskal, Gunhild; Heggeset, Tonje Marita Bjerkan; Nguyen, Giang-Son; Haugen, Tone; Jønsson, Malene; Egas, Conceição; Hidalgo, Aurelio; Wentzel, Alexander; Lewin, Anna (Peer reviewed; Journal article, 2022)Most bacteria live in bioflms in their natural habitat rather than the planktonic cell stage that dominates during traditional laboratory cultivation and enrichment schemes. The present study describes the establishment ... -
Health benefits of microalgae and their microbiomes
Krohn, Ines; Menanteau-Ledouble, Simon; Hageskal, Gunhild; Astafyeva, Yekaterina; Jouannais, Pierre; Nielsen, Jeppe Lund; Pizzol, Massimo; Wentzel, Alexander; Streit, Wolfgang R. (Peer reviewed; Journal article, 2022)Microalgae comprise a phylogenetically very diverse group of photosynthetic unicellular pro- and eukaryotic organisms growing in marine and other aquatic environments. While they are well explored for the generation of ... -
Intracellular metabolite pool changes in response to nutrient depletion induced metabolic switching in Streptomyces coelicolor
Wentzel, Alexander; Sletta, Håvard; Wellington, E.M.H.; Ellingsen, Trond Erling; Bruheim, Per (Journal article; Peer reviewed, 2012)A metabolite profiling study of the antibiotic producing bacterium Streptomyces coelicolor A3(2) has been performed. The aim of this study was to monitor intracellular metabolite pool changes occurring as strains of S. ... -
Isolation and genome sequencing of four Arctic marine Psychrobacter strains exhibiting multicopper oxidase activity
Shojaei Moghadam, Morteza; Albersmeier, Andreas; Winkler, Anika; Cimmino, Lorenzo; Rise, Kjersti; Hohmann-Marriott, Martin Frank; Kalinowski, Jörn; Rückert, Christian; Wentzel, Alexander; Lale, Rahmi (Journal article; Peer reviewed, 2016)Background Marine cold-temperature environments are an invaluable source of psychrophilic microbial life for new biodiscoveries. An Arctic marine bacterial strain collection was established consisting of 1448 individual ... -
Kinetic Study of Butanol Production from Mixtures of Glucose and Xylose and Investigation of Different Pre-growth Strategies
Birgen, Cansu; Berglihn, Olaf Trygve; Preisig, Heinz A.; Wentzel, Alexander (Journal article; Peer reviewed, 2019)This study proposes a dynamic model that describes key characteristics of fermentative butanol production from glucose and xylose mixtures. The model has 12 parameters and incorporates noncompetitive inhibitory interaction ... -
Microbial-induced calcium carbonate precipitation: An experimental toolbox for in situ and real-time investigation of micro-scale pH evolution
Zehner, Jennifer Simone; Røyne, Anja; Wentzel, Alexander; Sikorski, Pawel (Peer reviewed; Journal article, 2020)Concrete is the second most consumed product by humans, after water. However, the production of conventional concrete causes more than 5% of anthropogenic CO2 emissions and therefore there is a need for emission-reduced ... -
Modeling the Growth of Clostridium beijerinckii NCIMB 8052 on Lignocellulosic Sugars
Birgen, Cansu; Markussen, Sidsel; Wentzel, Alexander; Preisig, Heinz A.; Wittgens, Bernd (Peer reviewed; Journal article, 2018)To our knowledge, this is the first growth model of Clostridium beijerinckii NCIMB 8052 on glucose and xylose as representative lignocellulosic sugars, which considers the synergistic effects of sugars on the growth rate. ... -
New dienelactone hydrolase from microalgae bacterial community-Antibiofilm activity against fish pathogens and potential applications for aquaculture
Bergmann, Lutgardis; Le, Simone Balzer; Hageskal, Gunhild; Preuss, Lena; Han, Yuchen; Astafyeva, Yekaterina; Loevenich, Simon; Emmann, Sarah; Pérez-García, Pablo; Indenbirken, Daniela; Katzowitsch, Elena; Thümmler, Fritz; Alawi, Malik; Wentzel, Alexander; Streit, Wolfgang R.; Krohn, Ines (Peer reviewed; Journal article, 2024)Biofilms are resistant to many traditional antibiotics, which has led to search for new antimicrobials from different and unique sources. To harness the potential of aquatic microbial resources, we analyzed the meta-omics ... -
Novel archaeal thermostable cellulases from an oil reservoir metagenome
Lewin, Anna; Zhou, Jinglie; Pham, Vu Thuy Trang; Haugen, Tone; El Zeiny, Mohamed; Aarstad, Olav Andreas; Liebl, Wolfgang; Wentzel, Alexander; Liles, Mark R. (Peer reviewed; Journal article, 2017)Microbial assemblages were sampled from an offshore deep sub-surface petroleum reservoir 2.5 km below the ocean floor off the coast of Norway, providing conditions of high temperature and pressure, to identify new thermostable ... -
Optimized submerged batch fermentation strategy for systems scale studies of metabolic switching in Streptomyces coelicolor A3(2)
Wentzel, Alexander; Bruheim, Per; Øverby, Anders; Jakobsen, Øyvind Mejdell; Sletta, Håvard; Omara, Walid A.M.; Hodgson, David A.; Ellingsen, Trond Erling (Journal article; Peer reviewed, 2012)Systems biology approaches to study metabolic switching in Streptomyces coelicolor A3(2) depend on cultivation conditions ensuring high reproducibility and distinct phases of culture growth and secondary metabolite production. ... -
Predicting strain engineering strategies using iKS1317: a genome-scale metabolic model of Streptomyces coelicolor
Kumelj, Tjasa; Sulheim, Snorre; Wentzel, Alexander; Almaas, Eivind (Peer reviewed; Journal article, 2018)Streptomyces coelicolor is a model organism for the Actinobacteria, a phylum known to produce an extensive range of different bioactive compounds that include antibiotics currently used in the clinic. Biosynthetic gene ... -
Production of propionate using metabolically engineered strains of Clostridium saccharoperbutylacetonicum
Baur, Tina; Wentzel, Alexander; Dürre, Peter (Peer reviewed; Journal article, 2022)The carboxylic acid propionate is a valuable platform chemical with applications in various fields. The biological production of this acid has become of great interest as it can be considered a sustainable alternative to ... -
Response Surface Methodology for Understanding Glucose and Xylose Utilization by Clostridium beijerinckii NCIMB 8052
Birgen, Cansu; Markussen, Sidsel; Wentzel, Alexander; Preisig, Heinz A. (Peer reviewed; Journal article, 2018)We applied response surface methodology to understand the effect and extend of carbon catabolite repression (CCR) on growth of Clostridium beijerinckii NCIMB 8052 using xylose and glucose as representative lignocellulosic ...