Browsing SINTEF Open by Author "Skjåk-Bræk, Gudmund"
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Exploiting mannuronan C-5 epimerases in commercial alginate production
Tøndervik, Anne; Aarstad, Olav Andreas; Aune, Randi; Maleki, Susan; Rye, Philip D; Dessen, Arne; Skjåk-Bræk, Gudmund; Sletta, Håvard (Peer reviewed; Journal article, 2020) -
Identification of a Pivotal Residue for Determining the Block Structure-Forming Properties of Alginate C-5 Epimerases
Stanisci, Annalucia; Tøndervik, Anne; Gaardløs, Margrethe; Lervik, Anders; Skjåk-Bræk, Gudmund; Sletta, Håvard; Aachmann, Finn Lillelund (Peer reviewed; Journal article, 2020)Alginate is a linear copolymer composed of 1→4 linked β-d-mannuronic acid (M) and its epimer α-l-guluronic acid (G). The polysaccharide is first produced as homopolymeric mannuronan and subsequently, at the polymer level, ... -
Overall size of mannuronan C5-Epimerases influences their ability to epimerize modified alginates and alginate gels
Stanisci, Annalucia; Aarstad, Olav Andreas; Tøndervik, Anne; Sletta, Håvard; Dypås, Lene Brattsti; Skjåk-Bræk, Gudmund; Aachmann, Finn Lillelund (Peer reviewed; Journal article, 2018)A family of seven mannuronan C5-epimerases (AlgE1-AlgE7) produced by Azotobacter vinelandii is able to convert β-d-mannuronate (M) to its epimer α-l-guluronate (G) in alginates. Even sharing high sequence homology at the ... -
Strain Construction and Process Development for Efficient Recombinant Production of Mannuronan C-5 Epimerases in Hansenula polymorpha
Tøndervik, Anne; Aune, Randi; Degelmann, Adelheid; Piontek, Michael; Ertesvåg, Helga; Skjåk-Bræk, Gudmund; Sletta, Håvard (Peer reviewed; Journal article, 2022)Alginates are linear polysaccharides produced by brown algae and some bacteria and are composed of β-D-mannuronic acid (M) and α-L-guluronic acid (G). Alginate has numerous present and potential future applications within ...