Browsing SINTEF Open by Author "Klinkenberg, Geir"
Now showing items 1-17 of 17
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A high-throughput drug combination screen of targeted small molecule inhibitors in cancer cell lines
Flobak, Åsmund; Niederdorfer, Barbara; To, Vu; Thommesen, Liv; Klinkenberg, Geir; Lægreid, Astrid (Journal article; Peer reviewed, 2019)While there is a high interest in drug combinations in cancer therapy, openly accessible datasets for drug combination responses are sparse. Here we present a dataset comprising 171 pairwise combinations of 19 individual ... -
Cytotoxicity of poly(Alkyl cyanoacrylate) nanoparticles
Sulheim, Einar; Iversen, Tore Geir; To, Vu; Klinkenberg, Geir; Sletta, Håvard; Schmid, Ruth; Hatletveit, Anne Rein; Wågbø, Ane Marit; Sundan, Anders; Skotland, Tore; Sandvig, Kirsten; Mørch, Ýrr Asbjørg (Peer reviewed; Journal article, 2017)Although nanotoxicology has become a large research field, assessment of cytotoxicity is often reduced to analysis of one cell line only. Cytotoxicity of nanoparticles is complex and should, preferentially, be evaluated ... -
Differences in sperm functionality and intracellular metabolites in Norwegian Red bulls of contrasting fertility
Narud, Birgitte; Klinkenberg, Geir; Khezri, Abdolrahman; Zeremichael, Teklu Tewoldebrhan; Stenseth, Else-Berit; Nordborg, Anna; Haukaas, Tonje Husby; Morrell, Jane; Heringstad, Bjørg; Myromslien, Frøydis Deinboll; Kommisrud, Elisabeth (Peer reviewed; Journal article, 2020)In the dairy breeding industry, prediction of bull fertility in artificial insemination (AI) is important for efficient and economically sustainable production. However, it is challenging to identify bulls with superior ... -
Engineering chitinolytic activity into a cellulose-active lytic polysaccharide monooxygenase provides insights into substrate specificity
Jensen, Marianne Slang; Klinkenberg, Geir; Bissaro, Bastien; Chylenski, Piotr; Vaaje-Kolstad, Gustav; Kvitvang, Hans Fredrik Nyvold; Nærdal, Guro Kruge; Sletta, Håvard; Forsberg, Zarah; Eijsink, Vincent (Journal article; Peer reviewed, 2019)Lytic polysaccharide monooxygenases (LPMOs) catalyze oxidative cleavage of recalcitrant polysaccharides such as cellulose and chitin and play an important role in the enzymatic degradation of biomass. While it is clear ... -
Global Assessment of Mycobacterium avium subsp. hominissuis Genetic Requirement for Growth and Virulence
Dragset, Marte Singsås; Ioerger, Thomas R.; Loevenich, Maja; Haug, Markus; Sivakumar, Niruja; Marstad, Anne; Cardona, Pere-Joan; Klinkenberg, Geir; Rubin, Eric J.; Steigedal, Magnus; Flo, Trude Helen (Peer reviewed; Journal article, 2019) -
High-throughput assay for effect screening of amphotericin B and bioactive components on filamentous Candida albicans
Haslene-Hox, Hanne; Nærdal, Guro Kruge; Mørch, Ýrr Asbjørg; Hageskal, Gunhild; Tøndervik, Anne; Vikenes, Anette; Johnsen, Heidi; Klinkenberg, Geir; Sletta, Håvard (Peer reviewed; Journal article, 2022) -
High-throughput screening reveals higher synergistic effect of MEK inhibitor combinations in colon cancer spheroids
Folkesson, Evelina; Niederdorfer, Barbara; To, Vu; Thommesen, Liv; Klinkenberg, Geir; Lægreid, Astrid; Flobak, Åsmund (Peer reviewed; Journal article, 2020)Drug combinations have been proposed to combat drug resistance, but putative treatments are challenged by low bench-to-bed translational efficiency. To explore the effect of cell culture format and readout methods on ... -
Identification of novel cyanoacrylate monomers for use in nanoparticle drug delivery systems prepared by miniemulsion polymerisation – A multistep screening approach
Hyldbakk, Astrid; Mørch, Ýrr Asbjørg; Snipstad, Sofie; Åslund, Andreas; Klinkenberg, Geir; Nakstad, Vu; Wågbø, Ane Marit; Schmid, Ruth Baumberger; Molesworth, Peter Patrick (Peer reviewed; Journal article, 2022)Poly (alkyl cyanoacrylate) (PACA) polymeric nanoparticles (NPs) are promising drug carriers in drug delivery. However, the selection of commercially available alkyl cyanoacrylate (ACA) monomers is limited, because most ... -
Identification of Regulatory Genes and Metabolic Processes Important for Alginate Biosynthesis in Azotobacter vinelandii by Screening of a Transposon Insertion Mutant Library
Mærk, Mali; Jakobsen, Øyvind Mejdell; Sletta, Håvard; Klinkenberg, Geir; Tøndervik, Anne; Ellingsen, Trond Erling; Valla, Svein; Ertesvåg, Helga (Peer reviewed; Journal article, 2020)Azotobacter vinelandii produces the biopolymer alginate, which has a wide range of industrial and pharmaceutical applications. A random transposon insertion mutant library was constructed from A. vinelandii ATCC12518Tc in ... -
Interlaboratory evaluation of a digital holographic microscopy–based assay for label-free in vitro cytotoxicity testing of polymeric nanocarriers
Marzi, Anne; Eder, Kai Moritz; Barroso, Álvaro; Wågbø, Ane Marit; Mørch, Ýrr Asbjørg; Hatletveit, Anne Rein; Visnes, Torkild; Schmid, Ruth Baumberger; Klinkenberg, Geir; Kemper, Björn; Schnekenburger, Jürgen (Peer reviewed; Journal article, 2022)State-of-the-art in vitro test systems for nanomaterial toxicity assessment are based on dyes and several staining steps which can be affected by nanomaterial interference. Digital holographic microscopy (DHM), an ... -
Magnetic-Responsive Carbon Nanotubes Composite Scaffolds for Chondrogenic Tissue Engineering
Saranya, Muthusamy; da Silva, Aldeliane M.; Karjalainen, Hanna; Klinkenberg, Geir; Schmid, Ruth Baumberger; McDonagh, Birgitte Hjelmeland; Molesworth, Peter Patrick; Sigfúsdóttir, Margrét S.; Wågbø, Ane Marit; Santos, Susana. G.; Couto, Cristiana; Karjalainen, Ville-Pauli; Gupta, Shuvashis Das; Järvinen, Topias; de Roy, Luisa; Seitz, Andreas. M.; Finnilä, Mikko; Saarakkala, Simo; Haaparanta, Anne Marie; Janssen, Lauriane; Lorite, Gabriela S. (Peer reviewed; Journal article, 2023)The demand for engineered scaffolds capable of delivering multiple cues to cells continues to grow as the interplay between cell fate with microenvironmental and external cues is revealed. Emphasis has been given to develop ... -
Quality assessment of LNP-RNA therapeutics with orthogonal analytical techniques
Parot, Jérémie; Mehn, Dora; Jankevics-Jones, Hanna; Markova, Natalia; Carboni, Michele; Olaisen, Camilla; Hoel, Andrea Draget; Sigfúsdóttir, Margrét S.; Meier, Florian; Drexel, Roland; Vella, Gabriella; Mcdonagh, Birgitte Hjelmeland; Hansen, Terkel; Bui, Huong; Klinkenberg, Geir; Visnes, Torkild; Gioria, Sabrina; Urban-Lopez, Patricia; Prina-Mello, Adriele; Borgos, Sven Even F.; Caputo, Fanny; Calzolai, Luigi (Peer reviewed; Journal article, 2024)The availability of analytical methods for the characterization of lipid nanoparticles (LNPs) for in-vivo intracellular delivery of nucleic acids is critical for the fast development of innovative RNA therapies. In this ... -
Semen quality parameters including metabolites, sperm production traits and fertility in young Norwegian Red AI bulls
Narud, Birgitte; Khezri, Abdolrahman; Nordborg, Anna; Klinkenberg, Geir; Zeremichael, Teklu Tewoldebrhan; Stenseth, Else-Berit; Heringstad, Bjørg; Kommisrud, Elisabeth; Myromslien, Frøydis Deinboll (Peer reviewed; Journal article, 2022)Genomic selection in cattle breeding has gradually allowed younger bulls to be recruited for semen production. In this study, sperm quality parameters, seminal plasma and sperm metabolites, semen production capacity and ... -
Sperm quality parameters, fertilizing potential, metabolites, and DNA methylation in cold-stored and cryopreserved milt from Atlantic salmon (Salmo salar L.)
Narud, Birgitte; Khezri, Abdolrahman; Zeremichael, Teklu Tewoldebrhan; Eriksen, Anne-Lene; Grevle, Inger Synnøve; Nordborg, Anna; Klinkenberg, Geir; Wilson, Robert Charles; Kommisrud, Elisabeth (Journal article; Peer reviewed, 2023)Cold storage and freezing/thawing of milt may affect sperm functionality and the subsequent fertilization ability of milt. This study aimed to investigate sperm quality parameters and fertilization potential of Atlantic ... -
Standardization of an in vitro assay matrix to assess cytotoxicity of organic nanocarriers: a pilot interlaboratory comparison
Eder, Kai Moritz; Marzi, Anne; Wågbø, Ane Marit; Vermeulen, Jolanda P.; de la Fonteyne-Blankestijn, Liset J. J.; Rösslein, Matthias; Ossig, Rainer; Klinkenberg, Geir; Vandebriel, Rob J.; Schnekenburger, Jürgen (Peer reviewed; Journal article, 2022)Nanotechnologies such as nanoparticles are established components of new medical devices and pharmaceuticals. The use and distribution of these materials increases the requirement for standardized evaluation of possible ... -
Streptomyces tardus sp. nov.: A Slow-Growing Actinobacterium Producing Candicidin, Isolated From Sediments of the Trondheim Fjord
Králová, Stanislava; Sandoval-Powers, Megan; Fawwal, Dorelle V.; Degnes, Kristin Fløgstad; Lewin, Anna; Klinkenberg, Geir; Nguyen, Giang-Son; Liles, Mark R.; Wentzel, Alexander (Peer reviewed; Journal article, 2021)Marine environments are home to an extensive number of microorganisms, many of which remain unexplored for taxonomic novelty and functional capabilities. In this study, a slow-growing Streptomyces strain expressing unique ... -
Targeted siRNA lipid nanoparticles for the treatment of KRAS-mutant tumors
Anthiya, Shubaash; Oztürk, Süleyman Can; Yanik, Hamdullah; Tavukcuoglu, Ece; Sahin, Adem; Datta, Dhrubajyoti; Charisse, Klaus; Álvarez, David Moreira; Loza, María Isabel; Calvo, Alfonso; Sulheim, Einar; Loevenich, Simon; Klinkenberg, Geir; Schmid, Ruth Baumberger; Manoharan, Muthiah; Esendağlı, Güneş; Jose Alonso, Maria (Peer reviewed; Journal article, 2023)K-RAS is a highly relevant oncogene that is mutated in approximately 90% of pancreatic cancers and 20–25% of lung adenocarcinomas. The aim of this work was to develop a new anti-KRAS siRNA therapeutic strategy through the ...