Browsing SINTEF Open by Author "Brautaset, Trygve"
Now showing items 1-11 of 11
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A comparative analysis of the properties of regulated promoter systems commonly used for recombinant gene expression in Escherichia coli
Balzer, Simone; Kucharova, Veronika; Megerle, Judith; Lale, Rahmi; Brautaset, Trygve; Valla, Svein (Journal article; Peer reviewed, 2013)Background Production of recombinant proteins in bacteria for academic and commercial purposes is a well established field; however the outcomes of process developments for specific proteins are still often unpredictable. ... -
Composition analysis and minimal treatments to solubilize 6 polysaccharides from the brown seaweed Laminaria digitata 7 for microbial growth of thermophiles
Allahgoli, Leila; Sardari, Roya; Hakvåg, Sigrid; Ara, Kazi; Kristjansdottir, Thordis Anna; Aasen, Inga Marie; Fridjonsson, Olafur; Brautaset, Trygve; Hreggvidsson, Gudmundur O.; Nordberg Karlsson, Eva (Peer reviewed; Journal article, 2020)Brown macroalgae (Phaeophyta) hold high potential as feedstock for biorefineries due to high biomass productivity and carbohydrate content. They are, however, a challenging, unconventional feedstock for microbial refining ... -
Corrigendum: Genome-Based Genetic Tool Development for Bacillus methanolicus: Theta- and Rolling Circle-Replicating Plasmids for Inducible Gene Expression and Application to Methanol-Based Cadaverine Production
Irla, Marta Katarzyna; Heggeset, Tonje Marita Bjerkan; Nærdal, Ingemar; Paul, Lidia; Haugen, Tone; Le, Simone Balzer; Brautaset, Trygve; Wendisch, Volker F. (Journal article; Peer reviewed, 2019)Bacillus methanolicus is a thermophilic methylotroph able to overproduce amino acids from methanol, a substrate not used for human or animal nutrition. Based on our previous RNA-seq analysis a mannitol inducible promoter ... -
Draft genome sequence of the docosahexaenoic acid producing thraustochytrid Aurantiochytrium sp. T66
Liu, Bin; Ertesvåg, Helga; Aasen, Inga Marie; Vadstein, Olav; Brautaset, Trygve; Heggeset, Tonje Marita Bjerkan (Peer reviewed; Journal article, 2016)Thraustochytrids are unicellular, marine protists, and there is a growing industrial interest in these organisms, particularly because some species, including strains belonging to the genus Aurantiochytrium, accumulate ... -
Evaluation of Heterologous Biosynthetic Pathways for Methanol-Based 5-Aminovalerate Production by Thermophilic Bacillus methanolicus
Brito, Luciana Fernandes; Irla, Marta Katarzyna; Nærdal, Ingemar; Balzer Le, Simone; Delépine, Baudoin; Heux, Stéphanie; Brautaset, Trygve (Peer reviewed; Journal article, 2021)The use of methanol as carbon source for biotechnological processes has recently attracted great interest due to its relatively low price, high abundance, high purity, and the fact that it is a non-food raw material. In ... -
Methanol-based cadaverine production by genetically engineered Bacillus methanolicus strains
Nærdal, Ingemar; Pfeifenschneider, Johannes; Brautaset, Trygve; Wendisch, Volker F. (Peer reviewed; Journal article, 2015)Methanol is regarded as an attractive substrate for biotechnological production of value-added bulk products, such as amino acids and polyamines. In the present study, the methylotrophic and thermophilic bacterium Bacillus ... -
Methylotrophic Bacillus methanolicus encodes two chromosomal and one plasmid born NAD+ dependent methanol dehydrogenase paralogs with different catalytic and biochemical properties
Krog, Anne; Heggeset, Tonje Marita Bjerkan; Müller, Jonas E.N.; Kupper, Christiane E.; Schneider, Olha; Vorholt, Julia A.; Ellingsen, Trond Erling; Brautaset, Trygve (Journal article, 2013)Bacillus methanolicus can utilize methanol as the sole carbon source for growth and it encodes an NAD+-dependent methanol dehydrogenase (Mdh), catalyzing the oxidation of methanol to formaldehyde. Recently, the genomes of ... -
Non-Invasive Analysis of Recombinant mRNA Stability in Escherichia coli by a Combination of Transcriptional Inducer Wash-Out and qRT-PCR
Kucharova, Veronika; Aakvik, Trine; Almaas, Eivind; Naas, Adrian Ertsås; Brautaset, Trygve; Valla, Svein (Journal article; Peer reviewed, 2013)mRNA stability is one among many parameters that can potentially affect the level of recombinant gene expression in bacteria. Blocking of the entire prokaryotic transcription machinery by addition of rifampicin is commonly ... -
A novel expression system for lytic polysaccharide monooxygenases
Courtade, Gaston; Le, Simone Balzer; Sætrom, Gerd Inger; Brautaset, Trygve; Aachmann, Finn Lillelund (Peer reviewed; Journal article, 2017) -
Production of Value-Added Chemicals by Bacillus methanolicus Strains Cultivated on Mannitol and Extracts of Seaweed Saccharina latissima at 50C
Hakvåg, Sigrid; Nærdal, Ingemar; Heggeset, Tonje Marita Bjerkan; Kristiansen, Kåre Andre; Aasen, Inga Marie; Brautaset, Trygve (Peer reviewed; Journal article, 2020)The facultative methylotroph Bacillus methanolicus MGA3 has previously been genetically engineered to overproduce the amino acids L-lysine and L-glutamate and their derivatives cadaverine and γ-aminobutyric acid (GABA) ... -
Transcriptome analysis of thermophilic methylotrophic Bacillus methanolicus MGA3 using RNA-sequencing provides detailed insights into its previously uncharted transcriptional landscape
Irla, Marta; Neshat, Armin; Brautaset, Trygve; Rückert, Christian; Kalinowski, Jörn; Wendisch, Volker F. (Journal article; Peer reviewed, 2015)Background Bacillus methanolicus MGA3 is a thermophilic, facultative ribulose monophosphate (RuMP) cycle methylotroph. Together with its ability to produce high yields of amino acids, the relevance of this microorganism ...