Browsing SINTEF Open by Author "Aakvik, Trine"

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A new and improved host-independent plasmid system for RK2-based conjugal transfer

Aakvik, Trine; Lale, Rahmi; Degnes, Kristin Fløgstad; Lando, Malin; Valla, Svein (Journal article, 2014)

Bacterial conjugation is a process that is mediated either by a direct cell-to-cell junction or by formation of a bridge between the cells. It is often used to transfer DNA constructs designed in Escherichia coli to recipient ...
Non-Invasive Analysis of Recombinant mRNA Stability in Escherichia coli by a Combination of Transcriptional Inducer Wash-Out and qRT-PCR

Kucharova, Veronika; Aakvik, Trine; Almaas, Eivind; Naas, Adrian Ertsås; Brautaset, Trygve; Valla, Svein (Journal article; Peer reviewed, 2013)

mRNA stability is one among many parameters that can potentially affect the level of recombinant gene expression in bacteria. Blocking of the entire prokaryotic transcription machinery by addition of rifampicin is commonly ...