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dc.contributor.authorStanisci, Annalucia
dc.contributor.authorAarstad, Olav Andreas
dc.contributor.authorTøndervik, Anne
dc.contributor.authorSletta, Håvard
dc.contributor.authorDypås, Lene Brattsti
dc.contributor.authorSkjåk-Bræk, Gudmund
dc.contributor.authorAachmann, Finn Lillelund
dc.date.accessioned2020-12-28T13:56:20Z
dc.date.available2020-12-28T13:56:20Z
dc.date.created2017-10-20T15:06:03Z
dc.date.issued2018
dc.identifier.citationCarbohydrate Polymers. 2018, 180 256-263.en_US
dc.identifier.issn0144-8617
dc.identifier.urihttps://hdl.handle.net/11250/2721038
dc.description.abstractA family of seven mannuronan C5-epimerases (AlgE1-AlgE7) produced by Azotobacter vinelandii is able to convert β-d-mannuronate (M) to its epimer α-l-guluronate (G) in alginates. Even sharing high sequence homology at the amino acid level, they produce distinctive epimerization patterns. The introduction of new G-blocks into the polymer by in vitro epimerization is a strategy to improve the mechanical properties of alginates as biomaterial. However, epimerization is hampered when the substrate is modified or in the gelled state. Here it is presented how native and engineered epimerases of varying size perform on steric hindered alginate substrates (modified or as hydrogels). Reducing the size of the epimerases enables the epimerization of otherwise inaccessible regions in the alginate polymer. Even though the reduction of the size affects the productive binding of epimerases to the substrate, and hence their activity, the smaller epimerases could more freely diffuse into calcium-alginate hydrogel and epimerize it.en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.no*
dc.subjectChemically modified alginatesen_US
dc.subjectAlginate hydrogelsen_US
dc.subjectChemo-enzymatic strategiesen_US
dc.subjectMannuronan C5-epimerasesen_US
dc.subjectAlginateen_US
dc.titleOverall size of mannuronan C5-Epimerases influences their ability to epimerize modified alginates and alginate gelsen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionacceptedVersionen_US
dc.rights.holderThis is the authors’ accepted and refereed manuscript to the article.This manuscript version is made available under the CC-BY-NC-ND 4.0 license. Published article is available at ScienceDirect: https://www.sciencedirect.com/science/article/pii/S0144861717311360?via%3Dihuben_US
dc.source.pagenumber256-263en_US
dc.source.volume180en_US
dc.source.journalCarbohydrate Polymersen_US
dc.identifier.doi10.1016/j.carbpol.2017.09.094
dc.identifier.cristin1506317
dc.relation.projectNorges forskningsråd: 226244en_US
dc.relation.projectNorges forskningsråd: 264065en_US
dc.relation.projectNorges forskningsråd: 250875en_US
dc.relation.projectNorges forskningsråd: 221576en_US
cristin.unitcode7401,80,1,0
cristin.unitnameBioteknologi og nanomedisin
cristin.ispublishedtrue
cristin.fulltextpreprint
cristin.qualitycode1


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Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal
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