dc.contributor.author | Baier, Tobias | |
dc.contributor.author | Hansen-Hagge, Thomas | |
dc.contributor.author | Gransee, Rainer | |
dc.contributor.author | Crombe, A. | |
dc.contributor.author | Schmahl, S. | |
dc.contributor.author | Paulus, C. | |
dc.contributor.author | Drese, K.S. | |
dc.contributor.author | Keegan, H. | |
dc.contributor.author | Martin, C. | |
dc.contributor.author | O'Leary, J. J. | |
dc.contributor.author | Furuberg, Liv | |
dc.contributor.author | Solli, L. | |
dc.contributor.author | Grønn, P. | |
dc.contributor.author | Falang, I. | |
dc.contributor.author | Karlgård, Aina | |
dc.contributor.author | Gulliksen, Anja | |
dc.contributor.author | Karlsen, F | |
dc.date.accessioned | 2017-02-20T14:37:25Z | |
dc.date.available | 2017-02-20T14:37:25Z | |
dc.date.created | 2017-02-16T09:51:30Z | |
dc.date.issued | 2009 | |
dc.identifier.citation | Lab on a Chip. 2009, 3399-3405. | nb_NO |
dc.identifier.issn | 1473-0189 | |
dc.identifier.uri | http://hdl.handle.net/11250/2431477 | |
dc.description.abstract | A Lab-On-Chip system with an instrument is presented which is capable of performing total sample preparation and automated extraction of nucleic acid from human cell samples fixed in a methanol based solution. The target application is extraction of mRNA from cervical liquid based cytology specimens for detection of transformed HPV-infections. The device accepts 3 ml of sample and performs the extraction in a disposable polymer chip of credit card size. All necessary reagents for cell lysis, washing, and elution are stored on-chip and the extraction is performed in two filter stages; one for cell pre-concentration and the other for nucleic acid capture. Tests performed using cancer cell lines and cervical liquid based cytology specimens confirm the extraction of HPV-mRNA by the system. | |
dc.language.iso | eng | nb_NO |
dc.title | Hands-free sample preparation platform for nucleic acid analysis | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | nb_NO |
dc.source.pagenumber | 3399-3405 | nb_NO |
dc.source.journal | Lab on a Chip | nb_NO |
dc.identifier.doi | 10.1039/B910421F | |
dc.identifier.cristin | 1451118 | |
cristin.unitcode | 7401,90,31,0 | |
cristin.unitname | Mikrosystemer og nanoteknologi | |
cristin.ispublished | true | |
cristin.fulltext | postprint | |
cristin.qualitycode | 1 | |