Hands-free sample preparation platform for nucleic acid analysis
Baier, Tobias; Hansen-Hagge, Thomas; Gransee, Rainer; Crombe, A.; Schmahl, S.; Paulus, C.; Drese, K.S.; Keegan, H.; Martin, C.; O'Leary, J. J.; Furuberg, Liv; Solli, L.; Grønn, P.; Falang, I.; Karlgård, Aina; Gulliksen, Anja; Karlsen, F
Journal article, Peer reviewed
Permanent lenke
http://hdl.handle.net/11250/2431477Utgivelsesdato
2009Metadata
Vis full innførselSamlinger
- Publikasjoner fra CRIStin - SINTEF AS [5649]
- SINTEF Digital [2383]
Sammendrag
A Lab-On-Chip system with an instrument is presented which is capable of performing total sample preparation and automated extraction of nucleic acid from human cell samples fixed in a methanol based solution. The target application is extraction of mRNA from cervical liquid based cytology specimens for detection of transformed HPV-infections. The device accepts 3 ml of sample and performs the extraction in a disposable polymer chip of credit card size. All necessary reagents for cell lysis, washing, and elution are stored on-chip and the extraction is performed in two filter stages; one for cell pre-concentration and the other for nucleic acid capture. Tests performed using cancer cell lines and cervical liquid based cytology specimens confirm the extraction of HPV-mRNA by the system.